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2 edition of Factors affecting collagen gene expression in a human osteosarcoma cell line. found in the catalog.

Factors affecting collagen gene expression in a human osteosarcoma cell line.

Carl John Cresswell

Factors affecting collagen gene expression in a human osteosarcoma cell line.

by Carl John Cresswell

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Published by University of Manchester in Manchester .
Written in English


Edition Notes

Manchester thesis (M.Sc.), School of Biological Sciences.

ContributionsUniversity of Manchester. School of Biological Sciences.
The Physical Object
Pagination104p.
Number of Pages104
ID Numbers
Open LibraryOL16572874M

Uwe Michel, in International Review of Cytology, 5 BORG. Bone morphogenetic proteins (BMP/OP) are members of the transforming growth factor-β family; some of the family members are important for proper bone formation.A comparison of the gene expression pattern of the mouse myoblast cell line C2C12 treated with BMP OP-1 and an untreated control resulted in the identification of a gene. Our findings indicate that the COL11A2 collagen gene expression in Saos-2 osteosarcoma cells is mediated by EWS/ERG-responsive cis-elements. COL11A2 expression in osteosarcoma may be related to formation of chondroid extracellular matrix, which is one of the characteristics of the osteosarcoma, especially for its chondroblastic subset.

Collagen XXIV has been estimated to represent about 4% of the amount of collagen I in bone, thus slightly less than collagen V, the regulator of collagen I fibrillogenesis. The estimate was based on gene expression analyses that in addition documented coexpression of collagens I and XXIV genes at ossification centers in the mouse embryo (11). Background: Osteosarcoma, the most common malignant primary bone tumor, typically occurs during the adolescent growth spurt. Germ-line genetic variation in genes critical in growth regulation could confer altered risk of osteosarcoma. Methods: Fifty-two common single nucleotide polymorphisms (SNP) in 13 genes were genotyped in a prospective case-control study of osteosarcoma ( osteosarcoma.

Regulation of type I collagen synthesis by 1,dihydroxyvitamin D3 in human osteosarcoma cells.) J Biol Chem enhanced by dexamethasone in a mouse osteoblastic cell line alpha 2 beta 1 is a positive regulator of collagenase (MMP-1) and collagen alpha 1 (1) gene expression. J Biol Chem Other investigators have suggested that an amphiregulin may mediate in part an anabolic effect on osteoblasts. Using microassays to target gene expression profile changes in PTH-treated UMR osteoblast osteosarcoma cell line, amphiregulin (AR), a member of .


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Factors affecting collagen gene expression in a human osteosarcoma cell line by Carl John Cresswell Download PDF EPUB FB2

Methods: To identify cell lines that are well suited for studies of critical cancer-related phenotypes, such as tumour initiation, growth and metastasis, we have evaluated 22 osteosarcoma cell lines for in vivo tumorigenicity, in vitro colony-forming ability, invasive/migratory potential and proliferation capacity.

Importantly, we have also identified mRNA and microRNA (miRNA) gene expression Cited by:   c, d Gene expression in MC3T3-E1 cells expressing c-Fos-ER or mutant (inactive) c-Fos*-ER in the presence/absence of tamoxifen (c) and in the H2-c-fosLTR OS cell line (Fos Tg-C3) expressing IPTG Author: Kazuhiko Matsuoka, Latifa Bakiri, Lena I.

Wolff, Markus Linder, Amanda Mikels-Vigdal, Ana Patiño-Gar. In this study, the percent collagen synthesized by the cultures (collagen relative to total protein synthesis) was not reported; as a result, it was not possible to determine the selectivity of the 1,25(OH) 2 D 3 effect for collagen synthesis.

1,25(OH) 2 D 3 also increases collagen expression in the human osteoblastic osteosarcoma cell line MG. Chondrocyte isolation.

Human chondrocytes were isolated from the articular cartilage of femoral heads obtained at the time of surgery for total hip replacement (12 OA patients [4 men and 8 women], with a mean ± SD age of ± years) or for femoral neck fracture (10 control subjects [2 men and 8 women], with a mean ± SD age of ± years), as previously described ().Cited by:   The COL1A1 gene provides instructions for making part of a large molecule called type I collagen.

Collagens are a family of proteins that strengthen and support many tissues in the body, including cartilage, bone, tendon, skin, and the white part of the eye (the sclera). Type I collagen is the most abundant form of collagen in the human body.

Runx-2 and collagen type I are known to be upregulated by BMP-2 in human prostate cancer cells, osteosarcoma cells renal carcinoma cells (21,25,26).

Therefore, using quantitative RT-PCR, we found that Runx-2, Osx, ALP and collagen type I were significantly upregulated in sorted ALDH br and ALDH lo cells treated with BMP-2 at a concentration of.

During the tissue synthesis process, which requires an increased production of collagen, manganese is required for the activation of prolidase, an enzyme that functions to provide the amino acid proline, for collagen formation in human skin cells.

Nutritional co-factors required for tissue regeneration include zinc, vitamin C and amino acids. Abstract. Increasingly it is recognized that the extracellular matrix (ECM) plays a critical role in the normal development and differentiated phenotype of cells and tissues (Lee et al, ; Bokel et al, ).Engagement of ECM molecules by cells through surface receptors, including integrins, results in the activation of signaling pathways along with specific changes in gene expression.

Sp7 was expressed in both the 2 human osteosarcoma cell lines examined and has previously been found in the rat osteosarcoma cell line, ROS17/. Additionally, amplification of chromosomal region 12q that contains Sp7 has been noted in various human osteosarcoma [47, 48] suggesting that Sp7 may be involved in neoplastic disease.

Runx2 directly regulates Sp7 expression, and osteoblasts and bone formation are also absent in Sp7 –/– mice [23,24].As Runx2 is an upstream transcription factor of Sp7, Runx2 is expressed in Sp7 –/– mice [24,25].One major difference between Runx2 –/– mice and Sp7 –/– mice is the number of mesenchymal cells in the presumptive bone regions.

There are abundant mesenchymal cells. The basal ZIC1 expression in the human bone osteosarcoma cell line SAOS2 is much higher (>10 fold) than the level in normal stromal cells, i.e.

human skin fibroblasts. The differences in the ZIC1 expression mirrored closely the degree of ZIC1 promoter methylation in the two cell types, being considerably lower in SaOS2 cells than in the fibroblast. Systematic variation in gene expression patterns in human cancer cell lines.

Nat Genet ;– Properties and reactivity of a new human osteosarcoma cell line (HOS 58). The MC3T3‐E1 mouse calvaria‐derived cell line has been used to study the role of collagen synthesis in osteoblast differentiation. MC3T3‐E1 cells, like several previously characterized osteoblast culture systems, expressed osteoblast markers and formed a mineralized extracellular matrix.

Chondroblastic interface cells easily lost their potential to produce collagen genes in non-stretched conditions, rather than fibroblastic midsubstance cells. Uni-axial mechanical stretches increased the type I collagen gene expression of interface and midsubstance cells up to and 6-fold levels of each non-stretched control, respectively.

HACE1 was highly expressed in previously described osteoblast cell lines (OBB and OB1 (ref. 43)) compared to SJSA and SaOS-2 osteosarcoma cell lines (Fig. 1e), while HEK cell line. E-Mail Address. Password. Forgotten Password.

Remember Me. factors or cells and the network-forming capacity and anchoring function of certain collagen types could contribute to the formation of scaffolds promoting tissue repair or regeneration [2,5,6].

Collagens—the basic structural module The name ‘‘collagen’’ is used as a generic term for proteins forming a characteristic triple helix. Ghosh AK: Factors involved in the regulation of type I collagen gene expression: implication in fibrosis.

Exp Biol Med. – PubMed/NCBI. 23 Branton MH and Kopp JB: TGF-β and fibrosis. Microbes Infect. – 24 Blobe GC, Schiemann WP and Lodish HF: Role of transforming growth factor β in human disease.

Sp7 was expressed in both the 2 human osteosarcoma cell lines examined and has previously been found in the rat osteosarcoma cell line, ROS17/. Additionally, amplification of chromosomal region 12q that contains Sp7 has been noted in various human osteosarcoma [47,48] suggesting that Sp7 may be involved in neoplastic disease.

So far, lifestyle-related factors have not been linked to osteosarcomas in adults, either. Still, there are some factors that affect osteosarcoma risk.

Age. The risk of osteosarcoma is highest for those between the ages of 10 especially during the teenage growth spurt. The human osteosarcoma cell lines, Saos-2, MNNG/HOS (MNNG) and B and mouse osteosarcoma cell line, LM8, were cultured in Roswell Park Memorial Institute or Eagle’s minimum essential medium supplemented with 10% heat-inactivated fetal bovine serum (FBS), 50 U/ml penicillin and 50 μ g/ml streptomycin at 37°C in a humidified atmosphere.WT1 suppresses tumorigenicity of a Wilms' tumor cell line (Haber et al., ) as well as other cells such as osteosarcoma cells, CV1 cells and fibroblasts (Englert et al., ; Luo et al.,   Disseminated breast cancer cells often enter a state of dormancy in metastatic niches, and their reactivation causes the emergence of metastatic disease even many years later.

The tetraspanin TM4SF1 was previously identified as a molecule that promotes metastatic outgrowth in mice injected with dormant cancer cells.

Gao et al. found that TM4SF1 promotes the reactivation of dormant breast.